Nicking endonuclease-mediated isothermal exponential amplification for double-stranded DNA detection

- This approach was simple, only employing three simple primers for dsDNA detection.
- This approach realized true isothermal amplification and was a one-pot.
- The generality of this approach were proved by specific DNA from Vibrio parahemolyticus ATCC17802.
- This approach did not require thermal cycling instrument and shorten reaction time.