کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7146433 | 1462087 | 2014 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
MISFET-based biosensing interface for neurons guided growth and neuronal electrical activities recording
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
A hybrid circuit of a transistor-based chip was implemented and characterized for the neuronal electrical activity recording. The integration of microfluidic architectures was developed to control precisely neurites outgrowth and form topologically defined and stable neural networks. Individual neural cells from rat retinae and Lymnaea stagnalis snails were immobilized on gates regions of Metal Insulator Semiconductor Field Effect Transistors (MISFET). Neuronal orientation was achieved in both cases but neuronal action potentials were only recorded in the L. stagnalis case. They were successfully triggered and inhibited by implementing a picrotoxin - GABA - picrotoxin injection protocol, exhibiting a direct influence of picrotoxin on the “spike type” action potential waveform. The implementation of the whole process of neuronal culture and subsequent activity monitoring constitutes a proof-of-principle experiment for the development of neuroelectronic systems for signal processing studies adapted to low-density neuronal cultures.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 203, November 2014, Pages 375-381
Journal: Sensors and Actuators B: Chemical - Volume 203, November 2014, Pages 375-381
نویسندگان
F. Larramendy, A. Bendali, M.C. Blatché, F. Mathieu, S. Picaud, P. Temple-Boyer, L. Nicu,