کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7146867 1462087 2014 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Hierarchical mesoporous silica film modified near infrared SPR sensor with high sensitivities to small and large molecules
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Hierarchical mesoporous silica film modified near infrared SPR sensor with high sensitivities to small and large molecules
چکیده انگلیسی
Hierarchical mesoporous silica (meso-SiO2) films with 90 nm thickness and 4.34 nm interpore spacing were fabricated on the SPR sensor chips for rapid detection of small and large molecules. With non-adsorbing glucose molecules the refractive-index sensitivity of the sensor was detected to increase from 8283.2 nm/RIU to 22354 nm/RIU with moving the resonance wavelength from λR = 920 nm to λR = 1220 nm. Adsorption of CTAB molecules within the meso-SiO2 film leads to a more than 7 times enhancement in the sensor response, showing the Langmuir isotherm adsorption behavior. The sensor can also detect proteins larger than the pore size of the meso-SiO2 film, whose rough surface makes the sensor responses very close to those obtained with the uncoated SPR chips. The findings indicate that the nonspecific adsorption of goat anti-human IgG molecules on the meso-SiO2 film did not bring about obvious sterical hindrance to their proper specificity, enabling the sensor to detect human IgG at a concentration as low as 10 nM. The work demonstrated the outstanding applicability of the meso-SiO2 film coated SPR sensors for real-time and sensitive detection of both small and large molecules.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 203, November 2014, Pages 690-696
نویسندگان
, , , , ,