کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7146972 1462089 2014 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Virus purification and enrichment by hydroxyapatite chromatography on a chip
ترجمه فارسی عنوان
تصفیه و غنی سازی ویروس توسط کروماتوگرافی هیدروکسی آپاتیت بر روی تراشه
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
The spread of infectious diseases has become a global health concern. In order to diagnose infectious diseases quickly and accurately, next-generation DNA sequencing techniques for genetic analysis of infectious viruses have been developed rapidly. However, it takes a very long time to pretreat clinical samples for genetic analysis using next-generation sequencers. We have therefore developed a microfluidic chromatography chip that can purify and enrich viruses in a sample using hydroxyapatite particles packed in a micro-column. We demonstrated the purification of virus from a mixture of virus and FBS protein, and enrichment of the virus using this novel microfluidic chip.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 201, 1 October 2014, Pages 185-190
نویسندگان
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