کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7147029 | 1462091 | 2014 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A solid phase-bridge based DNA amplification technique with fluorescence signal enhancement for detection of cancer biomarkers
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: A solid phase-bridge based DNA amplification technique with fluorescence signal enhancement for detection of cancer biomarkers A solid phase-bridge based DNA amplification technique with fluorescence signal enhancement for detection of cancer biomarkers](/preview/png/7147029.png)
چکیده انگلیسی
This study aims to establish an improved solid phase DNA amplification technique for the detection of DNA biomarkers in the point of care (POC) based molecular diagnostic fields of human diseases including cancer. To date, a complex of DNA biomarkers is known to be associated with human diseases. In particular, many known biomarkers are associated with human cancers, and these need to be simultaneously tested for early detection and prognosis of cancer. In this study, we developed a DNA amplification technique with signal enhancement that combines with solid phase bridge PCR and fluorescence resonance energy transfer (FRET), which can be improved the multiple targets detection simultaneously without obtaining false-positives that caused by the primers binding to each other when multiple targets are amplifying on a solid surface. Here, we showed that the fluorescence signals are 2.5 times enhanced on the Au surface with the KRAS gene (target DNA), which is a common DNA biomarker of human cancers, compared to on the Au surface with non-target DNA by using the present technique. Furthermore, we confirmed the storage conditions of the Au sensor with the immobilized primers for the commercialization as a molecular diagnostic device in the field of POC (point of care)-based cancer applications. Therefore, the present technique will be useful not only for the detection of multiple targets simultaneously without any false-positive signal generated by primers, but also enable integration with a microfluidic system in order to minimize the platform for the genetic analysis of DNA biomarkers in the field of diagnostic applications for disease treatment.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 199, August 2014, Pages 220-225
Journal: Sensors and Actuators B: Chemical - Volume 199, August 2014, Pages 220-225
نویسندگان
Yong Shin, Jitae Kim, Tae Yoon Lee,