کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7147160 | 1462098 | 2014 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
GADH screen-printed biosensor for gluconic acid determination in wine samples
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
Gluconate dehydrogenase (GADH) biosensors have been developed for the satisfactory determination of gluconic acid in wine samples, without any pretreatment. The biosensors have been fabricated by cross-linking immobilization of GADH onto screen-printed carbon electrodes, containing the mediator tetrathiafulvalene (TTF). Chronoamperograms have been registered at +100 mV vs. screen-printed Ag/AgCl electrode by successive additions of a gluconic acid solution in the concentration range from 9.0 to 131.4 μM. This method shows a reproducibility of 8.1% (n = 3) related to the slopes of these calibration curves and a repeatability of 3.2% (n = 5). The procedure has shown an average capability of detection of 9.0 μM for a probability of false positive and negative of 0.05. Wine samples have been analyzed with these biosensors, obtaining satisfactory results.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 192, 1 March 2014, Pages 56-59
Journal: Sensors and Actuators B: Chemical - Volume 192, 1 March 2014, Pages 56-59
نویسندگان
Lorena del Torno-de Román, M. Asunción Alonso-Lomillo, Olga DomÃnguez-Renedo, Arrate Jaureguibeitia, M. Julia Arcos-MartÃnez,