کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7147242 | 1462099 | 2014 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A novel signal-on DNAzyme-based electrochemiluminescence, sensor for Pb2+
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
A novel signal-on electrochemiluminescence (ECL) biosensor for the determination of lead (Pb2+) was developed. Background suppression for the ECL detection of Pb2+ and hybridization in the presence of Pb2+ were realized using a hairpin probe. The 8-17 DNAzymes, which hybridized with their substrate strands, were immobilized onto the surface of a glassy carbon electrode (GCE) via amino coupling method. In the presence of Pb2+, the substrate strands were cleaved and released, while DNAzymes alone remained on the GCE surface. The single-stranded DNAzyme could hybridize with a hairpin probe containing an ECL luminophore resulting in quantitative ECL signals. The sequence of the hairpin probe was designed and optimized for hybridizing with single-stranded DNAzyme but not with DNAzyme-substrate double strands. This signal-on strategy possessed low ECL background signals, high sensitivity and selectivity toward Pb2+. The ECL sensing approach presented excellent response to Pb2+ from 10Â pM to 100Â nM with a detection limit of 6.4Â pM.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 191, February 2014, Pages 60-66
Journal: Sensors and Actuators B: Chemical - Volume 191, February 2014, Pages 60-66
نویسندگان
Yan-fang Wu, Zhi-min Cai, Geng-huang Wu, Ming-cong Rong, Ya-qi Jiang, Chao-yong James Yang, Xi Chen,