کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7147432 | 1462098 | 2014 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Microfluidic devices with integrated dual-capacitively coupled contactless conductivity detection to monitor binding events in real time
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Microfluidic devices with integrated dual-capacitively coupled contactless conductivity detection to monitor binding events in real time Microfluidic devices with integrated dual-capacitively coupled contactless conductivity detection to monitor binding events in real time](/preview/png/7147432.png)
چکیده انگلیسی
This paper describes the fabrication of microfluidic devices with integrated dual capacitively coupled contactless conductivity detection (C4D) for monitoring binding events. The avidin-biotin model was employed to show the feasibility of the C4D as a detection mode. The binding event was monitored using microfluidic channels fabricated in poly(dimethylsiloxane) (PDMS). Detection electrodes were fabricated in a three-electrode arrangement by sputtering a Ti/Au/Ti layer over a glass wafer, which were electrically insulated by a SiO2 layer. The dielectric layer over the sensing electrode area was chemically modified with biotin. After introducing avidin solutions on microchannels, binding events were detected on conductivity sensorgrams by applying a 300-kHz-sinusoidal waveform with amplitude of 2 V (peak-to-peak) to the exciting electrode. The electrical characterization of the modified electrode indicates that the conductivity signal is changed due to the alteration on the dielectric constant of the surface. The limit of detection for avidin was 1.5 μg mLâ1, representing 23 nmol Lâ1.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 192, 1 March 2014, Pages 239-246
Journal: Sensors and Actuators B: Chemical - Volume 192, 1 March 2014, Pages 239-246
نویسندگان
Wendell Karlos Tomazelli Coltro, Rodrigo de Santis Neves, Artur de Jesus Motheo, José Alberto Fracassi da Silva, Emanuel Carrilho,