کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7147434 | 1462099 | 2014 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Spatially reinforced Au nano-cavities as a reaction nano-reservoir for trace analysis of DNA hybridization
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
Spatially reinforced Au nano-cavities (SR-nAu) with reduced tip-to-tip displacement create strongly localized surface plasmon resonance by Raman scattering. Within SR-nAu, the nano-cavity structure with high aspect ratio was optimized as the nano-reservoir for the distinction of DNA hybridization responses from specific DNA sequences of Avian influenza virus. In the nano-reservoir, the suspended or immobilized probe, its complementary and noncomplementary target sequences were characterized at very low concentration through enhanced Raman spectra. The presence of Raman shift at 738Â cmâ1, the characteristic breathing mode of thymine, and the change of relative peaks intensity at 1603 and 1554Â cmâ1 before and after hybridization were particularly taken as the major indications to validate the match or mismatch state of DNA sequences. Through a labeling-free means, and from the evidence provided by specific complementary and noncomplementary DNA sequences, the recognition capability of nano-reservoir can be down to subnano-molar concentration (10â10Â M).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 191, February 2014, Pages 219-226
Journal: Sensors and Actuators B: Chemical - Volume 191, February 2014, Pages 219-226
نویسندگان
Chih-Kai Yao, Jiunn-Der Liao, Chih-Heng Lin, Yuh-Shyong Yang, Sheng-Hong Yu, Jung-Wei Yang,