کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7149156 | 1462117 | 2012 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Design and synthesis of fluorescent probe for high-sensitivity protein detection based on merocyanine structure and application to rapid electrophoretic gel stain
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
A new fluorescent molecular probe, sodium 3-((E)-2-((E)-3-(4-(dicyanomethylene)-6-methyl-4H-pyran-2-yl)allylidene)quinolin-1(2H)-yl)propane-1-sulfonate (1), possessing the cyanopyranyl moieties and merocyanine structure was designed and synthesized to detect proteins in solution and for SDS-PAGE. Compound 1 exhibited no fluorescence in the absence of proteins; however, it exhibited strong fluorescence on the addition of bovine serum albumin (BSA) as a result of polarity change by BSA. Compared with the conventional protocols for in-gel protein staining, such as SYPRO Ruby and silver staining, compound 1 achieves higher sensitivity, even though it offers a simplified, higher-throughput protocol. In fact, the total time required for protein staining was 60-90Â min under optimum conditions much shorter than that required by the less-sensitive silver staining or SYPRO Ruby staining protocols. Thus, compound 1 may facilitate high-sensitivity protein detection, and it may be widely applicable as a convenient tool in various scientific and medical fields.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 173, October 2012, Pages 114-119
Journal: Sensors and Actuators B: Chemical - Volume 173, October 2012, Pages 114-119
نویسندگان
Yoshio Suzuki, Kenji Yokoyama,