کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7149168 | 1462114 | 2013 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Label-free aptamer sensor based on silicon microring resonators
ترجمه فارسی عنوان
سنسور آتتامر بدون برچسب بدون استفاده از تشدید کننده سیلیکون میکروینگ
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
We present a label-free DNA aptamer sensor based on silicon microring resonators for the detection of human immunoglobulin E (IgE) and human thrombin. An array of silicon resonators consisting of three sensor microrings and one reference microring was fabricated and its surface sensitivity, detection limit and repeatability were investigated by the deposition of poly (sodium-4-styrenesulfonate) (PSS) and poly (allylamine hydrochloride) (PAH) multilayers. The surface sensitivity and the limit of detection of the fabricated sensor ring are found to be 0.35 nm/ng mmâ2 and 26 pg/mm2 in air and 0.24 nm/ng mmâ2 and 38 pg/mm2 in water, respectively. We have demonstrated quantitative, sensitive and specific detection of human IgE and thrombin with aptamer modified silicon microring resonators over a broad range of antigen concentrations. The sensor array showed an experimental detection limit of 33 pM and 1.4 nM for IgE and thrombin, respectively. Multiplexing was also performed successfully with a mixed solution of IgE and thrombin and proved the sensor to be highly specific. We believe our silicon microring resonator technology in combination with aptamers offers a low cost solution for various point-of-care applications.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 176, January 2013, Pages 552-559
Journal: Sensors and Actuators B: Chemical - Volume 176, January 2013, Pages 552-559
نویسندگان
Mi Kyoung Park, Jack Sheng Kee, Jessie Yiying Quah, Vivian Netto, Junfeng Song, Qing Fang, Eric Mouchel La Fosse, Guo-Qiang Lo,