کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7149734 1462117 2012 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Gold nanoparticles dispersed in zwitterionic surfactant for peroxidase immobilization in biosensor construction
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Gold nanoparticles dispersed in zwitterionic surfactant for peroxidase immobilization in biosensor construction
چکیده انگلیسی
In this study, peroxidase from alfalfa sprout extract (Medicago sativa) was immobilized on a gold electrode, using a solution of the zwitterionic surfactant 3-(1-tetradecyl-3-imidazolio)propanesulfonate (ImS3-14) containing well-dispersed gold nanoparticles. In the presence of hydrogen peroxide, the peroxidase enzyme catalyzes the oxidation of hydroquinone to o-quinone, which is electrochemically reduced at a potential of +0.09 V vs. Ag/AgCl. The hydroquinone biosensor exhibited excellent electrocatalytic activity under the optimized experimental conditions ([peroxidase] = 97 units mL−1, [H2O2] = 7.4 × 10−5 M, pH 7.0, frequency of 100 Hz, pulse amplitude of 60 mV and scan increment of 4.0 mV). Peak current linearity was observed in the range of 6.96 × 10−7 to 8.54 × 10−6 M, and the detection limit was estimated to be 1.88 × 10−7 M. This biosensor demonstrated good repeatability and reproducibility with relative standard deviations of 3.6% and 6.5%, respectively, and an acceptable stability (at least 100 determinations). The method has been applied to the determination of hydroquinone in skin-brightening creams and the recoveries were 98.3-104.5%.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 173, October 2012, Pages 483-490
نویسندگان
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