The System of Automatic Seedling Selection by the Early Molecular Diagnosis of Circadian Rhythms

We have developed the system of automatic seedling selection using an early molecular diagnosis of circadian rhythms. To measure the rhythms of gene expression by bioluminescence with in non-destructive and in real time, we used a measurement system for bioluminescence, called Kondotron. Moreover, to improve the efficiency of seedling selection, a manipulator for automatic seedling selection using the decision for the circadian rhythms in bioluminescence was introduced. Using this manipulator, it became possible to select automatically seedlings in the early stage based on the result of bioluminescent measurement. This system can measure 40 samples simultaneously more than one week in real time. Moreover, it is possible to measure seedlings continuously because new seedlings are supplied to measurement sample holders when they are picked out from the measurement holder. Therefore, it is possible to research in the flexible and wide experiment of gene expression since it can analysis the circadian rhythms under various purposes (automatic selection based on statistical processing, selection by threshold, and so on) and environment (light conditions) by this system.