Optimization of Recombinant Enzyme Production with Pichia pastoris in an Integrated Industrial Scale-down Production Plant

A scale down version of a multi component production plant, including a cell breeding reactor, a protein production reactor and a disc clarifier for enzyme separation is combined to an Integrated Bioprocess. Fully automated and global observable multistage parallel process courses are developed using industrial process control systems and atline measurements for enzyme concentration and enzyme activity. Optimal production conditions were found by application of DoE - Design of Experiment.