Simple Metabolic Modelling of Vero Cell Growth on Glucose in Fixed-bed Bioreactors

A novel metabolic model is formulated to simulate Vero cell growth on glucose in fixed-bed bioreactors by simplification of glucose fluxes in the central carbon metabolism. Model formulation is based on three designated metabolism states: respiratory metabolism, overflow metabolism and critical metabolism. The model is validated by experimental culture results and successfully predicts the dynamics of cell growth, glucose consumption and lactate production. As well as quantitatively describing glucose overflow metabolism in mammalian cell culture, the proposed model will be helpful for directly monitoring and controlling metabolism state of cells in order to achieve process optimization.