3D profile reconstruction of biological sample by in-line image-plane phase-shifting digital microscopic holography

To improve the measuring accuracy is an important research content for digital microscopic holography (DMH) development and application. In this study, we have upgraded application of DMH through the in-line image-plane phase-shifting technique and the image correlation algorithm to reconstruct the 3D profile of a biological sample. Importantly, since this novel DMH system can obtain the phase-shifting hologram with a high ratio of signal to noise conveniently, the reconstructed algorithm of DMH and the compensation operation of the phase aberration are simplified significantly. Moreover, by using the image correlation algorithm, the digital phase mask with high precision also can be obtained easily; thus both the measuring accuracy of DMH and the quality of the reconstructed image are improved significantly. More importantly, this kind of in-line image-plane phase-shifting digital microscopic holography provides a powerful imaging tool to simultaneously reconstruct the amplitude and the phase of the measured object with submicron scale resolution.

► Combination of the in-line image-plane phase-shifting technique with the image correlation algorithm.
► Reconstructed algorithm of digital holographic microscopy is simplified significantly.
► A kind of rapid compensation approach of phase aberration of digital holographic microscopy.
► Quality of the reconstructed image of digital holographic microscopy is improved significantly.