کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
735823 | 1461747 | 2006 | 16 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Use of confocal laser scanning microscopy (CLSM) for depthwise resolved microscale-particle image velocimetry (μ-PIV) Use of confocal laser scanning microscopy (CLSM) for depthwise resolved microscale-particle image velocimetry (μ-PIV)](/preview/png/735823.png)
A novel use of confocal laser scanning microscopy (CLSM) makes the truly focused field-of-view with well-defined depthwise resolution possible for microscale particle image velocimetry (μ-PIV) applications. The operating principle of the CLSM is presented using the point spread function (PSF) that describes diffracted images of extremely small particles. The implemented high-speed CLSM system using a Nipkow rotating disk is applied to measure the microscale rotating Couette flow field confined between two parallel horizontal disks that are 180-μm apart, with the bottom one stationary and the top one rotating and seeded by 200-nm fluorescent spheres. The CLSM provides much distinct particle images in comparison with the conventional wide-field microscopy (WFM) and the measured vector profiles are more concentric and accurate depicting closer to an ideal Couette flow.
Journal: Optics and Lasers in Engineering - Volume 44, Issues 3–4, March–April 2006, Pages 208–223