کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
739300 | 894074 | 2006 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Read-out concepts for multiplexed bead-based fluorescence immunoassays on centrifugal microfluidic platforms Read-out concepts for multiplexed bead-based fluorescence immunoassays on centrifugal microfluidic platforms](/preview/png/739300.png)
We present novel concepts to process and read out multiplexed, bead-based fluorescence immunoassays. At the start of the read-out process, a statistically arranged monolayer of color-encoded beads is aggregated in a detection chamber. Each bead is first identified by incorporated color tags which are either dyes or luminescing quantum dots (QDs). Subsequently, the reaction-specific fluorescence signal is quantified. The read-out process is accelerated by an in-house-developed image-processing algorithm. The optical read-out device consists of standard components, e.g. a color CCD-camera as detection unit, an LED as light source, optical filters, and a drive to spin the polymer disk. The liquid handling along the complete assay protocol is realized on a centrifugal lab-on-a-disk platform. We successfully demonstrate the performance of this device by the implementation of a hepatitis A and a tetanus assay.
Journal: Sensors and Actuators A: Physical - Volume 126, Issue 2, 14 February 2006, Pages 455–462