A highly selective affinity adsorption imprinting phosphorescence sensor for determination of trace alkaline phosphatase and prediction of human diseases

Polyamide membrane-wheat germ agglutinin-poly vinyl alcohol-affinity adsorption imprinting (abbreviated to PAM-WGA-PVA-AAI) was prepared using alkaline phosphatase (AP) as the template. The cavity in PAM-WGA-PVA-AAI not only matched with AP very well, but also had a sensitive response to AP. The Morin-SiO2-AbAP was obtained using Morin-SiO2 to label AbAP (goat anti human AP antibody). When AbAP-Morin-SiO2 was added to PAM-WGA-PVA-AAI, PAM-AP-AbAP-Morin-SiO2 formed by the immunoreaction between AbAP and AP in PAM-WGA-PVA-AAI due to the affinity between AbAP and AP was stronger than that between AP and WGA. The product could emit room temperature phosphorescence (RTP) because of the heavy atom effect of Pb2+. Motivated by the sensitive response of cavity in PAM-WGA-PVA-AAI to AP, a new PAM-WGA-PVA-AAI phosphorescence sensor for determination of trace AP and prediction of human diseases has been developed using PAM-WGA-PVA-AAI technique. The proposed sensor was sensitive (the detection limit (DL): 0.18 ag spot−1, corresponding concentration: 7.2 × 10−17 g mL−1 or 7.2 × 10−19 mol L−1), simple, rapid and highly selective, and it has been applied to the determination of trace AP in human serum and the forecast of human diseases, with the results agreeing well with those obtained by enzyme-linked immunoassay (ELISA). Meanwhile, the mechanism of this PAM-WGA-PVA-AAI phosphorescence sensor was discussed also.