کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
740461 1462108 2013 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Sensitive electrochemical detection of hydroxyl radical based on MBs–DNA–AgNPs nanocomposite
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Sensitive electrochemical detection of hydroxyl radical based on MBs–DNA–AgNPs nanocomposite
چکیده انگلیسی

A sensitive electrochemical method for the detection of hydroxyl radical (OH) was successfully developed based on magnetic beads (MBs)–DNA–Ag nanoparticles (AgNPs) (MBs–DNA–AgNPs) nanocomposite. MBs were utilized for bioseparation and AgNPs as signal indicator. When OH broke the DNA strands, AgNPs were detached from the surface of the MBs–DNA–AgNPs nanocomposite. Magnetic particles could be easily removed from the solution and the detached AgNPs were collected. Therefore, the anodic stripping voltammetry (ASV) signal of dissolved silver on glassy carbon (GC) electrode depended on the amounts of OH. The results show that the ASV signal linearly increases with the increase of the concentration of OH in the range of 0.05–4 μM, and the detection limit is as low as 10 nM, which is much lower than the detection limit obtained on the recently developed electrochemical and photoelectrochemical methods. In addition, the developed method has potential application in the screening of antioxidants.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 182, June 2013, Pages 504–509
نویسندگان
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