ORMOSIL oxygen sensors on polystyrene microplate for dissolved oxygen measurement

Oxygen sensors prepared from tetramethyl orthosilicate and dimethoxy dimethylsilane with tris(4,7-diphenyl-1,10-phenanthroline)ruthenium (II) as the sensing dye were coated onto the well bottom surface of a 96-well polystyrene microtiter plate to give a high-throughput system for dissolved oxygen measurement. The oxygen sensors give linear Stern–Volmer calibration plots, and produce reliable and reproducible results in the determination of IC50 values of drugs on a yeast model.