کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
742324 | 1462113 | 2013 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
One-pot preparation of uricase–poly(thiophene-3-boronic acid)–Ptnano composites for high-performance amperometric biosensing of uric acid
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Chemical oxidation of glycoprotein-bound thiophene-3-boronic acid (TBA) monomer by relatively mild oxidant chloroplatinate in neutral aqueous solution containing uricase yielded uricase–PTBA–Ptnano bionanocomposite with high enzymatic specific activity on a platinized (Ptplate) Au electrode, which was then covered by an outer-layer chitosan (CS) film to fabricate a CS/uricase–PTBA–Ptnano/Ptplate/Au electrode. This electrode exhibited a linear amperometric response to uric acid concentration from 5 μM to 1.2 mM with a sensitivity of 134 μA mM−1 cm−2, a limit of detection (S/N = 3) of 1 μM, and excellent operation/storage stability, which also worked well in serum samples.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 177, February 2013, Pages 116–123
Journal: Sensors and Actuators B: Chemical - Volume 177, February 2013, Pages 116–123
نویسندگان
Yi Huang, Lijuan Bu, Wen Wang, Xiaoli Qin, Zou Li, Zhao Huang, Yingchun Fu, Xiaoli Su, Qingji Xie, Shouzhuo Yao,