کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
742440 | 1462119 | 2012 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Continuous cell from cell separation by traveling wave dielectrophoresis
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
In this contribution we present a label-free continuous separation device for suspended-grown biological cells based on traveling wave dielectrophoresis (twDEP). Suspended-grown cells could otherwise only be separated by flow-cytometry which requires elaborate labeling steps. The chip consists of a structured PDMS layer on glass in which the channels have been defined. Parallel electrodes positioned along the microfluidic channel were used to expose selected cells to a twDEP force perpendicular to a pressure driven flow. With this device we have successfully separated viable Saccharomyces cerevisiae and Jurkat (T-cell leukemia) cells from a mix also containing cell culture debris, non-viable cells, and Lactobacillus casei bacteria.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 170, 31 July 2012, Pages 207–214
Journal: Sensors and Actuators B: Chemical - Volume 170, 31 July 2012, Pages 207–214
نویسندگان
Sander van den Driesche, Vivek Rao, Dietmar Puchberger-Enengl, Wojciech Witarski, Michael J. Vellekoop,