کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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742905 | 1462096 | 2014 | 6 صفحه PDF | دانلود رایگان |

In this paper, a label-free electrochemical impedance biosensor has been developed for protein detection based on terminal protection of small molecule linked-DNA. This strategy was demonstrated using folate (FA) and its binding protein folate receptor (FR) as a model case. In the present terminal protection system, with the increase of FR, it could protect more FA linked-DNA from hydrolysis by exonuclease I (Exo I), resulting in an increased electron transfer resistance response. By taking the advantage of the terminal protection assay, this label-free electrochemical biosensor achieved highly sensitive and selective detection of FR with a detection limit of 3 pM and a linear range from 10 pM to 500 nM, which was lower than some previously reported methods. Moreover, the label-free method did not need specific labelling and showed sequence-independent with DNA probes. Thus, it may offer a simple, cost-effective, highly selective and sensitive platform for electrochemical impedance detection of small molecule–protein interactions in clinical diagnostics.
Journal: Sensors and Actuators B: Chemical - Volume 194, April 2014, Pages 478–483