کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
743708 894369 2008 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Chemiluminescence imaging assay dipyridamole based on molecular imprinted polymer as recognition material
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Chemiluminescence imaging assay dipyridamole based on molecular imprinted polymer as recognition material
چکیده انگلیسی

A simple, sensitive and specific method has been developed for high throughput detection of dipyridamole. The proposed method is based on chemiluminescence (CL) imaging assay combined molecularly imprinted polymer (MIP) recognition. Molecularly imprinted microspheres were prepared using precipitation polymerization with methacrylic acid (MAA) as functional monomer, trimethylolpropane trimethacrylate (TRIM) as the crosslinker and dipyridamole as the template. Polymer as the artificial biomimetic recognition element for dipyridamole was coated in 96-microtiter well plates with poly(vinyl alcohol) (PVA) as glue. The amount of polymer-bound dipyridamole was determination based on dipyridamole peroxyoxalate chemiluminescence (PO-CL) reaction. The emitting light was measured with a high-resolution charge couple device (CCD) camera. Influencing factors were investigated and optimized in detail. The proposed method in this paper exhibits high selectivity and sensitivity to dipyridamole. Under the optimum conditions, the relative CL imaging intensity (AVG) is proportional to the concentration of dipyridamole ranging from 0.02 to 10 μg/ml. The detection limit is 0.006 μg/ml. The method can perform 96 independent measurements simultaneously in 30 min. These results show that the MIP-based CL imaging method can become a useful analytical technology for quick detection of dipyridamole in real sample.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 133, Issue 1, 28 July 2008, Pages 40–45
نویسندگان
, ,