کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
744017 | 1462123 | 2011 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Enhanced kinase sensing by radio-phosphorylation and oriented immobilization of a GBP-linked kinase substrate on a gold surface
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A new strategy for enhanced sensing of kinases was developed using radio-phosphorylation and a polypeptide with an affinity for gold that was linked directly to kinase substrates. Gold-binding polypeptide (GBP)-linked GST-fusion substrates were efficiently immobilized on gold-coated slides, which is highly desirable for protein chips. The novel fusion substrates permitted oriented substrate immobilization on gold-coated slides, thereby enhancing the sensitivity of kinase assays. Thus, the proposed strategy is applicable to the development of protein microarrays and microfluidic lab-on-a-chip (LOC) sensing the activities of protein kinases.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 160, Issue 1, 15 December 2011, Pages 632–636
Journal: Sensors and Actuators B: Chemical - Volume 160, Issue 1, 15 December 2011, Pages 632–636
نویسندگان
Jong Kook Rho, Jung Ae Kang, Mi Hee Choi, Kyong-Cheol Ko, Sang Hyun Park,