کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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744058 | 1462126 | 2008 | 8 صفحه PDF | دانلود رایگان |
Understanding the mechanism of how biological reactions produce mechanical loadings is fundamental to biomedical developments. A CMOS biosensor chip is developed to measure in situ the induced surface stress change by DNA hybridization. For 20-mer thiol-modified single stranded DNA (ssDNA), the mechanism of ssDNA attached to gold surface via a sulfur–gold linkage can be investigated by using the Langmuir adsorption model. Experimental results indicate that the immobilization response is less than 1 s, the total number of ssDNA molecules on the cantilever is about 3 × 1011, and the induced surface stress is 0.15 N/m. The surface stress sensitivity of the sensor is about 3.5 × 10−5 m/N. The estimated adsorption rate of the ssDNA is 0.005 s−1. The biosensor is capable of discriminating complimentary molecular targets and thus may provide a powerful platform for high throughput real-time analysis of DNA.
Journal: Sensors and Actuators B: Chemical - Volume 130, Issue 2, 28 March 2008, Pages 674–681