کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
744776 894402 2010 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Software-based quantitation of bioassays on CD
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Software-based quantitation of bioassays on CD
چکیده انگلیسی

Compact discs (CDs) can be used as rapid, low-cost, high-capacity screening platforms for running bioassays with no modification of the detection hardware (conventional standard optical drive). We describe herein a new protocol to read and quantitate biotin–streptavidin binding assays with a standard optical drive by using a CD-data analysis software (IsoBuster), which identifies erroneous sectors by locating the exact error position bit-by-bit and allows various data formats to be used. The numbers of erroneous sectors increase as a function of the streptavidin concentration in the tested solutions. High spatial accuracy and detection sensitivity (0.3 μg/mL) were achieved.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 148, Issue 2, 15 July 2010, Pages 620–623
نویسندگان
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