کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
745374 894419 2006 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Electrochemical characteristics of heme proteins in hydroxyethylcellulose film
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Electrochemical characteristics of heme proteins in hydroxyethylcellulose film
چکیده انگلیسی

A stable film made from hydroxyethylcellulose (HEC) on pyrolytic graphite (PG) electrode was employed for incorporating hemoglobin (Hb), myoglobin (Mb) and horseradish peroxidase (HRP), and the electrochemical characteristics of the proteins were studied correspondingly. Experimental results revealed that HEC film could greatly accelerate electron transfer between the proteins and electrode, and the proteins showed a thin layer electrochemical behavior in the film. Moreover, all the proteins in the film exhibited good catalytic activity towards the reduction of hydrogen peroxide (H2O2) in the low H2O2 concentration range. In the high concentration range, H2O2 would exhibit toxicity effect on the proteins. The electrochemical properties and electrocatalytic abilities of the three heme proteins in HEC film have been compared, and the optimal conditions for H2O2 biosensor fabrication have been obtained.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 113, Issue 1, 17 January 2006, Pages 106–111
نویسندگان
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