کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
745913 894435 2007 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Development of bi-enzyme microbiosensor based on solid-contact ion-selective microelectrodes for protein detection
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Development of bi-enzyme microbiosensor based on solid-contact ion-selective microelectrodes for protein detection
چکیده انگلیسی

A novel electrochemical microbiosensor for protein determination based on an all-solid-state microelectrodes with a Ppy [3,3-Co(1,2-C2B9H11)2] solid internal contact and immobilized trypsin and carboxypeptidase B was developed. The specificity of the sensor signal was investigated using artificial ester and amide substrates of the proteinases: Nα-benzoyl-l-arginine ethyl ester hydrochloride (BAEE), Nα-benzoyl-dl-arginine-p-nitroanilide hydrochloride (BAPNA), and Hippuryl-Arg. Study on a possibility of protein detection showed that quantitative proteins determination can be performed with the microbiosensor in the concentration range of 1–60 μg/ml.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 123, Issue 2, 21 May 2007, Pages 1096–1100
نویسندگان
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