کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
747148 894500 2006 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Platinum nanoparticle-modified carbon fiber ultramicroelectrodes for mediator-free biosensing
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Platinum nanoparticle-modified carbon fiber ultramicroelectrodes for mediator-free biosensing
چکیده انگلیسی

A novel platinum nanoparticle-modified carbon fiber ultramicroelectrode (Pt/CFUME) is proposed for the construction of a mediator-free amperometric ultramicroelectrode biosensor. The platinum nanoparticles electrodeposited on the ultramicroelectrodes greatly increase the surface areas with enhanced electron transfer characteristics between the electroactive centers of enzymes and the ultramicroelectrodes. By using horseradish peroxidase (HRP) as an enzyme model, we construct a mediator-free amperometric ultramicroelectrode HRP sensor for detecting H2O2. The resultant biosensor well displays the direct electrochemical behavior of HRP on the Pt/CFUME and the electrocatalytic response to the reduction of H2O2 without the aid of electron mediators. The steady-state response current is linearly related to the H2O2 concentration in the range of 0.64 μM–3.6 mM with a detection limit of 0.35 μM (S/N = 3) and a correlation coefficient of 0.9953.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 119, Issue 1, 24 November 2006, Pages 295–301
نویسندگان
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