کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
747411 | 894521 | 2006 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Planar optical sensors: A tool for screening enzyme activity in high density cell arrays
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Planar optical sensors: A tool for screening enzyme activity in high density cell arrays Planar optical sensors: A tool for screening enzyme activity in high density cell arrays](/preview/png/747411.png)
چکیده انگلیسی
Directed evolution has become a widely used technique in enzyme development. It leads often to vast libraries of potential new variants which have to be examined. High throughput methods offer the possibility to examine huge amounts of sample in parallel. Therefore sensor platforms for new high throughput applications are in demand. We want to introduce a two-dimensional sensor platform which can be used with a variety of fluorescence dyes to screen enzymatic reactions. In this paper, we want to introduce an approach for screening micro colony chips of E. coli for enhanced esterase activity with fluoresceine as indicator and for enhanced choline oxidase activity with pO2 sensitive particles as indicator.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 114, Issue 2, 26 April 2006, Pages 984–994
Journal: Sensors and Actuators B: Chemical - Volume 114, Issue 2, 26 April 2006, Pages 984–994
نویسندگان
Jochen Gerlach, Brigitte Pohn, Wolfgang Karl, Marcel Scheideler, Martina Uray, Horst Bischof, Helmut Schwab, Ingo Klimant,