کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
749930 894861 2007 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A single cell electrophysiological analysis device with embedded electrode
موضوعات مرتبط
مهندسی و علوم پایه شیمی الکتروشیمی
پیش نمایش صفحه اول مقاله
A single cell electrophysiological analysis device with embedded electrode
چکیده انگلیسی

A single cell electrophysiological analysis device with a sub-micrometer opening on embedded recording electrode has been demonstrated using a combined micro- and nano-machining process. This technique can reduce the series pipette resistance while keeping high seal resistance for electrophysiological measurements. In the prototype demonstration, the electrolyte pipette resistance is reduced to 200 kΩ using the microfabricated electrophysiological system with opening size of 500 nm in diameter. A 6 GΩ seal resistance is achieved on a HeLa cell, a cervical cancer cell line with size between 10 and 20 μm, on the same device. In a second experiment, the measured electrolyte pipette resistance is 1 MΩ using different pipette solution on a device with 800 nm opening and a 500 MΩ seal resistance is achieved on a vacuolar yeast cell of 3–5 μm in size.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators A: Physical - Volume 134, Issue 1, 28 February 2007, Pages 20–26
نویسندگان
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