کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
750479 1462067 2016 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Novel fluorescent ESIPT probe based on flavone for nitroxyl in aqueous solution and serum
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Novel fluorescent ESIPT probe based on flavone for nitroxyl in aqueous solution and serum
چکیده انگلیسی


• A ESIPT turn-on metal-free fluorescent probe for sensing nitroxyl (HNO) was developed.
• Large emission spectral shift from weak 404 nm to strong 520 nm in responding to HNO, as a consequence of the ESIPT turn-on.
• The probe exhibits a selective response to HNO over other biological reductants in solution.
• The probe was applied for quantitative detection of HNO in bovine serum with satisfactory results.

A excited-state intramolecular proton transfer (ESIPT) fluorescent turn-on probe (FLA-1) for the detection of nitroxyl (HNO) is designed and synthesized. It consists of a flavone derivative moiety as the fluorophore, linked via an ester moiety to a diphenylphosphinobenzoyl group as a recognition receptor, which forms an aza-ylide upon reaction with HNO. Intramolecular attack of the aza-ylide on the ester carbonyl group releases a fluorescent flavone derivative. In addition, the results showed that FLA-1 exhibited a selective response to HNO over other biological reductants and can quantitatively detect HNO in the range of 0–20 μM with the detection limit of 1.28 × 10−7 M in aqueous solution. Importantly, FLA-1 was successfully applied to detect HNO in the range of 2–20 μM with the detection limit of 3.96 × 10−7 M in complex biological samples (serum) demonstrating its value in practical application.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 224, 1 March 2016, Pages 209–216
نویسندگان
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