A high performance electrochemical sensor for NADH based on graphite nanosheet modified electrode

The electrocatalytical oxidation of dihydronicotinamide adenine dinucleotide (NADH) based on exfoliated graphite nanosheet (GNS) modified glassy carbon (GC) electrode was firstly explored. The GNSs/GC electrode showed favorable electron transfer for NADH oxidation and the oxidative peak potential was decreased to +0.32 V vs. Ag/AgCl. The electrochemical sensor based on the GNSs/GC electrode exhibited very high sensitivity of 535.0 mA M−1 cm−2, wide dynamic range from 2 μM to 4.69 mM and good stability for the detection of NADH, which are comparable to or better than those of previously reported most carbon nanomaterials modified electrodes, including recent graphene modified electrodes. Combining the low cost of GNSs with ready preparation from graphite, GNSs would be extremely attractive for numerous dehydrogenase-based bioelectrochemical devices such as biosensors, biofuel cells, and bioreactors in the future work.