کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
751419 | 895230 | 2010 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Amperometric hydrogen peroxide biosensor based on covalent immobilization of horseradish peroxidase on ferrocene containing polymeric mediator Amperometric hydrogen peroxide biosensor based on covalent immobilization of horseradish peroxidase on ferrocene containing polymeric mediator](/preview/png/751419.png)
In this work, a new type of amperometric hydrogen peroxide biosensor was fabricated for the determination of H2O2. Horseradish peroxidase (HRP) was immobilized on a glassy carbon electrode by poly(glycidyl methacrylate-co-vinylferrocene) (poly(GMA-co-VFc)) film. A polymeric electron transfer mediator, containing copolymers of glycidyl methacrylate (GMA) and vinylferrocene (VFc) with different molar ratios, was prepared by free-radical copolymerization. The amperometric response was measured as a function of H2O2 concentration, at a fixed potential of +0.35 V vs. Ag/AgCl in phosphate-buffered saline (pH 7.0). The mediated hydrogen peroxide biosensor showed a fast response of less than 4 s of linear range 2.0–30.0 mM, with a detection of 2.6 μM. The sensitivity of the biosensor for H2O2 was 10.42 nA/mM cm2.
Journal: Sensors and Actuators B: Chemical - Volume 145, Issue 1, 4 March 2010, Pages 444–450