کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
751477 | 895233 | 2009 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Preliminary studies of application of eggshell membrane as immobilization platform in sandwich immunoassay Preliminary studies of application of eggshell membrane as immobilization platform in sandwich immunoassay](/preview/png/751477.png)
A novel immunoassay using the eggshell membrane as immobilization platform for determination of human IgG (HIgG) has been developed. The immunoassay was based on a sandwich immunoreaction of rabbit anti-human IgG (primary antibody), HIgG and the goat anti-human IgG labeled with FITC (secondary antibody). Due to well permeability and highly biocompatibility of eggshell membrane, a friendly microenvironment was constructed to prolong the life-times of the immobilized proteins. Under the optimal conditions, the fluorescence intensity is linear to the concentration of HIgG in a range of 20–100 ng mL−1 (r = 0.9918). The proposed method was successfully applied to the determination of the HIgG in the standard blood serum, and the results are satisfactory compared with that obtained by general immunonephelometric method.
Journal: Sensors and Actuators B: Chemical - Volume 140, Issue 1, 18 June 2009, Pages 200–205