کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7556583 1491291 2018 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Determination of glyphosate residues in Egyptian soil samples
ترجمه فارسی عنوان
تعیین ضایعات گلیفواسات در نمونه های خاک مصر
کلمات کلیدی
گلیفوزات، آنتیبادی پلی کلنال، آنزیم ایمونوسیورسنت وابسته به آنزیم، کروماتوگرافی مایع با فشار بالا، نمونه های خاک، باقی مانده،
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
A sensitive linker-assisted enzyme-linked immunosorbent assay (L'ELISA) was developed for the analysis of glyphosate in Egyptian soil samples. Polyclonal glyphosate antibodies were produced from rabbits immunized with glyphosate protein conjugate. The conjugate was prepared by activating the carboxylic groups of proteins; thyroglobulin or bovine serum albumin with 1-ethyl-3- (3-diaminopropyl) carbodiimide hydrochloride and N-hydroxysulfosuccinimide followed by directly coupled to the amino group of glyphosate. The L'ELISA used succinic anhydride to derivatize glyphosate, which mimics the epitopic attachment of glyphosate to thyroglobulin. L'ELISA recognized the derivatized glyphosate with a limit of detection (LOD) of 0.8 ng g−1 and sensitivity (IC50 value) of 0.018 μg g−1. The recovery values of the spiked soil samples with different concentrations of glyphosate were in the range of 87.4-97.2%. Good correlation was achieved between L'ELISA and conventional high-pressure liquid chromatography (HPLC) with fluorescence detection. This study demonstrated the utility and convenience of the sensitive, simple, practical and cost-effective L'ELISA method for glyphosate analysis in soil samples. Also, it is ideal for rapid screening of a large number of environmental samples.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 557, 15 September 2018, Pages 1-6
نویسندگان
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