کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7556593 | 1491291 | 2018 | 32 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Use of DNA aptamer for sandwich type detection of Listeria monocytogenes
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
A single stranded (ss) DNA aptamer, specific to members of Listeria genus, was used to develop a two-site binding sandwich assay for capture and detection of L. monocytogenes. Antibody-immobilized immunomagnetic beads were used to capture L. monocytogenes, followed by their exposure to the aptamer detector. Detection was achieved by amplification of cell-bound aptamers by qPCR. The lower limit of detection for the combined assay was 2.5â¯CFU L. monocytogenes in 500â¯Î¼l buffer. This is juxtaposed to a detection limit of 2.4 log10â¯CFU in 500â¯Î¼l buffer for immunomagnetic separation coupled with qPCR detection of L. monocytogenes targeting the hly gene. When applied to turkey deli meat, subjected to 24â¯h of non-selective enrichment, the two-site binding sandwich assay showed positive results at initial inoculum levels of 1-2 log10â¯CFU per 25â¯g sample. Because of its lower limit of detection, the assay reported here could be useful for detection of L. monocytogenes in foods and environmental samples.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 557, 15 September 2018, Pages 27-33
Journal: Analytical Biochemistry - Volume 557, 15 September 2018, Pages 27-33
نویسندگان
Soo Hwan Suh, Soo Jung Choi, Hari P. Dwivedi, Matthew D. Moore, Blanca I. Escudero-Abarca, Lee-Ann Jaykus,