کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7556658 1491292 2018 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Modified parallel strategies for preparation of heteroduplex plasmids for in vitro mismatch repair assays
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Modified parallel strategies for preparation of heteroduplex plasmids for in vitro mismatch repair assays
چکیده انگلیسی
We present efficient and reproducible parallel strategies for preparing large quantities of pure heteroduplex plasmids containing defined mismatches. The strategies described involve the use of synthetic oligonucleotides, the commercially available pGEM-T plasmid, and nicking enzymes to prepare prerequisite ssDNA. Alternatively, bacterial packaging cell lines containing an engineered phagemid construct to produce ssDNA without the need of a helper phage were utilized, hence providing added flexibility and choice. These integrated approaches help to construct different mismatch substrates of choice in large quantities, thus enhancing the usability of mismatch repair assays and extending their range and accessibility to wider research groups.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 556, 1 September 2018, Pages 35-39
نویسندگان
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