کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7556686 | 1491293 | 2018 | 29 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Fabrication of glycerol biosensor based on co-immobilization of enzyme nanoparticles onto pencil graphite electrode
ترجمه فارسی عنوان
تولید بیوسنسور گلیسرول براساس همزمانی نانوذرات آنزیمی بر روی الکترود گرافیت مداد
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Glycerol kinase (GK) and glycerol-3- phosphate oxidase (GPO) nanoparticles (NPs) were prepared, characterized and immobilized onto pencil graphite (PG) electrode to fabricate an improved amperometric glycerol biosensor (GKNPs/GPONPs/PGE). GKNPs/GPONPs/PGE worked in optimum conditions of pH 7.0, temperature 30â¯Â°C, at an applied potential of â0.3â¯V. The biosensor exhibited wide linear response in a concentration range of glycerol (0.01-45â¯mM) with detection limit 0.0001â¯Î¼M. The biosensor revealed high sensitivity (7.24â¯Î¼AmMâ1cmâ2), low response time (2.5s) and a good agreement with the standard enzymic colorimetric method with a correlation coefficient (R2â¯=â¯0.99). The evaluation study of biosensor offered a good analytical recovery of 98.73% when glycerol concentration was added to the sera sample. In addition, within and between batches study of working electrode showed coefficients of variation as 0.105% and 0.14%, respectively. The application of biosensor is performed in the serum of apparently healthy subject and patients affected by cardiogenic shock. There was a 20% loss in initial activity of biosensor after its regular use over a time period of 180 days, while being stored at 4â¯Â°C.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 555, 15 August 2018, Pages 94-103
Journal: Analytical Biochemistry - Volume 555, 15 August 2018, Pages 94-103
نویسندگان
Vinay Narwal, C.S. Pundir,