کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7556805 | 1491297 | 2018 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Analysis of respiratory capacity in brain tissue preparations: high-resolution respirometry for intact hippocampal slices
ترجمه فارسی عنوان
تجزیه و تحلیل ظرفیت تنفسی در آماده سازی بافت مغزی: تنفس با وضوح بالا برای تکه های هیپوکامپ نابالغ
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کلمات کلیدی
تنفس با وضوح بالا، میزان مصرف اکسیژن، تکه های هیپوکامپ کامل، بیو انرژی الکتریکی، فسفوریلاسیون اکسیداتیو،
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
The evaluation of mitochondrial function provides the basis for the study of brain bioenergetics. However, analysis of brain mitochondrial respiration has been hindered by the low yield associated with mitochondria isolation procedures. Furthermore, isolating mitochondria or cells results in loss of the inherent complexity of the central nervous system. High-resolution respirometry (HRR), is a valuable tool to study mitochondrial function and has been used in diverse biological preparations ranging from isolated mitochondria to tissue homogenates and permeabilized tissue biopsies. Here we describe a novel methodology for evaluation of mitochondrial respiration using tissue preparations from the central nervous system, namely acute hippocampal slices from rodents, with HRR. By using acute intact hippocampal slices, tissue cytoarchitecture, intercellular communication and connectivity are preserved. Mitochondrial respiration was evaluated by using an adapted substrate-uncoupler-inhibitor titration (SUIT) protocol and the expected responses were observed. This methodology can be used to detect differences in mitochondrial function at the oxidative phosphorylation level and for studies with different brain oxidative substrates in physiological and neuropathological settings, by using a system that better represents the in vivo conditions than isolated mitochondria and/or cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 551, 15 June 2018, Pages 43-50
Journal: Analytical Biochemistry - Volume 551, 15 June 2018, Pages 43-50
نویسندگان
Cândida Dias, Cátia F. Lourenço, Rui M. Barbosa, João Laranjinha, Ana Ledo,