کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7556836 1491299 2018 32 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Analyzing mitochondrial function in human peripheral blood mononuclear cells
ترجمه فارسی عنوان
تجزیه و تحلیل عملکرد میتوکندری در سلول های تک سلولی خون محیطی انسان
کلمات کلیدی
یکپارچه عملکرد میتوکندری، فسفوریلاسیون اکسیداتیو، فعالیت پیچیده زنجیره ای حمل و نقل الکترون، لنفوسیت انسانی، سلول های تک هسته ای خون محیطی، بیو انرژی الکتریکی،
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Mitochondrial oxidative phosphorylation (OXPHOS) is responsible for producing most of the adenosine triphosphate required by eukaryotic cells. Lymphocytes make up the majority of the peripheral blood mononuclear cells. Peripheral blood mononuclear cells are readily obtainable, providing an ideal sample to monitor systemic changes and understand molecular signaling mechanisms in disease processes. Mitochondrial energy metabolism of lymphocyte has been used to screen for OXPHOS disorders. While there are increasing studies of lymphocyte OXPHOS, few studies examined activity of electron transport chain of lymphocyte mitochondria. We present an optimal protocol to harvest fresh peripheral blood mononuclear cells from human whole blood, determine integrated mitochondrial function, and analyze electron transport chain complex activity. Analyzing integrated mitochondrial function using OXPHOS provides data to uncover defects in the transport of substrates into the mitochondria, generation of reducing equivalents, the electron transport chain, and coupling to the production of adenosine triphosphate. The optimal conditions to harvest peripheral blood mononuclear cells were using blood anticoagulated with ethylenediaminetetraacetic acid, processed utilizing Lymphoprep™, and washed in phosphate buffered saline, all at room temperature. Using isolated peripheral blood mononuclear cells, integrated mitochondrial function and the activities of electron transport chain were determined.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 549, 15 May 2018, Pages 12-20
نویسندگان
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