کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7556862 | 1491298 | 2018 | 20 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Bio-electrochemical microelectrode arrays for glutamate and electrophysiology detection in hippocampus of temporal lobe epileptic rats
ترجمه فارسی عنوان
آرایه های میکرو الکترودهای بیو الکتروشیمیایی برای تشخیص گلوتامات و الکتروفیزیولوژی در هیپوکامپ موش های صرعی لوب تمپورال
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Temporal Lobe Epilepsy (TLE) is a chronic neurological disorder, characterized by sudden, repeated and transient central nervous system dysfunction. For better understanding of TLE, bio-nanomodified microelectrode arrays (MEA) are designed, for the achievement of high-quality simultaneous detection of glutamate signals (Glu) and multi-channel electrophysiological signals including action potentials (spikes) and local field potentials (LFPs). The MEA was fabricated by Micro-Electro-Mechanical System fabrication technology and all recording sites were modified with platinum black nano-particles, the average impedance decreased by nearly 90 times. Additionally, glutamate oxidase was also modified for the detection of Glu. The average sensitivity of the electrode in Glu solution was 1.999â¯Â±â¯0.032â¯Ãâ¯10â2pA/μM·μm2(nâ¯=â¯3) and linearity was Râ¯=â¯0.9986, with a good selectivity of 97.82% for glutamate and effective blocking of other interferents. In the in-vivo experiments, the MEA was subjected in hippocampus to electrophysiology and Glu concentration detection. During seizures, the fire rate of spikes increases, and the interspike interval is concentrated within 30â¯ms. The amplitude of LFPs increases by 3 times and the power increases. The Glu level (4.22â¯Î¼M, nâ¯=â¯4) was obviously higher than normal rats (2.24â¯Î¼M, nâ¯=â¯4). The MEA probe provides an advanced tool for the detection of dual-mode signals in the research of neurological diseases.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 550, 1 June 2018, Pages 123-131
Journal: Analytical Biochemistry - Volume 550, 1 June 2018, Pages 123-131
نویسندگان
Ziyue Li, Yilin Song, Guihua Xiao, Fei Gao, Shengwei Xu, Mixia Wang, Yu Zhang, Fengru Guo, Jie Liu, Yang Xia, Xinxia Cai,