کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7556939 1491300 2018 21 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Lectin-carbohydrate complex evaluation by chemiluminescence
ترجمه فارسی عنوان
ارزیابی پیچیده لکتین-کربوهیدرات با استفاده از شیمی معدنی
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
In order to characterize the affinity between specific carbohydrate-binding proteins such as lectins, a model is proposed to study these interactions using a polysaccharide membrane to simulate such adsorption. Here, lectin-carbohydrate interactions were chemiluminescently investigated using lectins conjugated to acridinium ester (AE) and polysaccharides composed of their respective specific carbohydrates. The lectin-AE conjugates were incubated with discs (0.0314-0.6358 cm2) of phytagel, chitosan and carrageenan. The complex formation chemiluminescently detected followed the Langmuir isotherm from which constants were estimated. The association constant (Ka) and maximum binding sites on the membranes were 2.4 × 10−7 M−1 ± 0.8 × 10−7 M−1 and 1.3 × 10−3 mol. mg−1 ± 0.3 × 10−3 mol. mg−1 (Con A); 0.9 × 10−6 M−1 ± 0.4 × 10−6 M−1 and 0.021 × 10−3 mol. mg−1 ± 0.003 × 10−3 mol. mg−1 (WGA) and 2.0 × 10−6 M−1 ± 0.9 × 10−6 M−1 and 0.069 × 10−3 mol. mg−1 ± 0.010 × 10−3 mol. mg−1 (PNA). The proposed model might be useful to study binding affinity and estimate the amount of binding not limited by the sugar content in the membrane.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 548, 1 May 2018, Pages 91-95
نویسندگان
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