کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7556939 | 1491300 | 2018 | 21 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Lectin-carbohydrate complex evaluation by chemiluminescence
ترجمه فارسی عنوان
ارزیابی پیچیده لکتین-کربوهیدرات با استفاده از شیمی معدنی
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
In order to characterize the affinity between specific carbohydrate-binding proteins such as lectins, a model is proposed to study these interactions using a polysaccharide membrane to simulate such adsorption. Here, lectin-carbohydrate interactions were chemiluminescently investigated using lectins conjugated to acridinium ester (AE) and polysaccharides composed of their respective specific carbohydrates. The lectin-AE conjugates were incubated with discs (0.0314-0.6358â¯cm2) of phytagel, chitosan and carrageenan. The complex formation chemiluminescently detected followed the Langmuir isotherm from which constants were estimated. The association constant (Ka) and maximum binding sites on the membranes were 2.4â¯Ãâ¯10â7â¯Mâ1â¯Â±â¯0.8â¯Ãâ¯10â7â¯Mâ1 and 1.3â¯Ãâ¯10â3â¯mol. mgâ1 ± 0.3â¯Ãâ¯10â3â¯mol. mgâ1 (Con A); 0.9â¯Ãâ¯10â6â¯Mâ1â¯Â±â¯0.4â¯Ãâ¯10â6â¯Mâ1 and 0.021â¯Ãâ¯10â3â¯mol. mgâ1 ± 0.003â¯Ãâ¯10â3â¯mol. mgâ1 (WGA) and 2.0â¯Ãâ¯10â6â¯Mâ1â¯Â±â¯0.9â¯Ãâ¯10â6â¯Mâ1 and 0.069â¯Ãâ¯10â3â¯mol. mgâ1 ± 0.010â¯Ãâ¯10â3â¯mol. mgâ1 (PNA). The proposed model might be useful to study binding affinity and estimate the amount of binding not limited by the sugar content in the membrane.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 548, 1 May 2018, Pages 91-95
Journal: Analytical Biochemistry - Volume 548, 1 May 2018, Pages 91-95
نویسندگان
Luiza Rayanna Amorim de Lima, Lúcia PatrÃcia Bezerra Gomes da Silva, Sinara Mônica Vitalino de Almeida, Thiago Barbosa Cahú, Eduardo Isidoro Carneiro Beltrão, Luiz Bezerra de Carvalho Júnior,