کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7556975 1491302 2018 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A simple and effective method for detecting precipitated proteins in MALDI−TOF MS
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
A simple and effective method for detecting precipitated proteins in MALDI−TOF MS
چکیده انگلیسی
MALDI−TOF MS has developed rapidly into an essential analytical tool for the life sciences. Cinnamic acid derivatives are generally employed in routine molecular weight determinations of intact proteins using MALDI−TOF MS. However, a protein of interest may precipitate when mixed with matrix solution, perhaps preventing MS detection. We herein provide a simple approach to enable the MS detection of such precipitated protein species by means of a “direct deposition method” -- loading the precipitant directly onto the sample plate. It is thus expected to improve routine MS analysis of intact proteins.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 546, 1 April 2018, Pages 1-4
نویسندگان
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