کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | ترجمه فارسی | نسخه تمام متن |
---|---|---|---|---|---|
7557855 | 1491355 | 2016 | 5 صفحه PDF | سفارش دهید | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Application of a non-hazardous vital dye for cell counting with automated cell counters
ترجمه فارسی عنوان
استفاده از یک رنگ حیاتی غیر خطرناک برای شمارش سلول با شمارنده سلول های اتوماتیک
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Recent advances in automated cell counters enable us to count cells more easily with consistency. However, the wide use of the traditional vital dye trypan blue (TB) raises environmental and health concerns due to its potential teratogenic effects. To avoid this chemical hazard, it is of importance to introduce an alternative non-hazardous vital dye that is compatible with automated cell counters. Erythrosin B (EB) is a vital dye that is impermeable to biological membranes and is used as a food additive. Similarly to TB, EB stains only nonviable cells with disintegrated membranes. However, EB is less popular than TB and is seldom used with automated cell counters. We found that cell counting accuracy with EB was comparable to that with TB. EB was found to be an effective dye for accurate counting of cells with different viabilities across three different automated cell counters. In contrast to TB, EB was less toxic to cultured HL-60Â cells during the cell counting process. These results indicate that replacing TB with EB for use with automated cell counters will significantly reduce the hazardous risk while producing comparable results.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 492, 1 January 2016, Pages 8-12
Journal: Analytical Biochemistry - Volume 492, 1 January 2016, Pages 8-12
نویسندگان
Soo In Kim, Hyun Jeong Kim, Ho-Jae Lee, Kiwon Lee, Dongpyo Hong, Hyunchang Lim, Keunchang Cho, Neoncheol Jung, Yong Weon Yi,
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