کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7558005 | 1491357 | 2015 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Evaluation of diffusion coefficients by means of an approximate steady-state condition in sedimentation velocity distributions
ترجمه فارسی عنوان
ارزیابی ضرایب انتشار با استفاده از حالت تقریبی حالت پایدار در توزیع سرعت رسوب
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کلمات کلیدی
ضریب انتشار، سرعت رسوب یون سانتریفوژ،
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
This investigation examined the feasibility of manipulating the rotor speed in sedimentation velocity experiments to spontaneously generate an approximate steady-state condition where the extent of diffusional spreading is matched exactly by the boundary sharpening arising from negative s-c dependence. Simulated sedimentation velocity distributions based on the sedimentation characteristics for a purified mucin preparation were used to illustrate a simple procedure for determining the diffusion coefficient from such steady-state distributions in situations where the concentration dependence of the sedimentation coefficient, s = s0/(1 + Kc), was quantified in terms of the limiting sedimentation coefficient as c â 0 (s0) and the concentration coefficient (K). Those simulations established that spontaneous generation of the approximate steady state could well be a feature of sedimentation velocity distributions for many unstructured polymer systems because the requirement that KcoÏ2s0/D be between 46 and 183 cmâ2 is not unduly restrictive. Although spontaneous generation of the approximate steady state is also a theoretical prediction for structured macromolecular solutes exhibiting linear concentration dependence of the sedimentation coefficient, s = s0(1 â kc), the required value of k is far too large for any practical advantage to be taken of this approach with globular proteins.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 490, 1 December 2015, Pages 20-25
Journal: Analytical Biochemistry - Volume 490, 1 December 2015, Pages 20-25
نویسندگان
David J. Scott, Stephen E. Harding, Donald J. Winzor,