کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7558246 | 1491364 | 2015 | 32 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Simple and sensitive progesterone detection in human serum using a CdSe/ZnS quantum dot-based direct binding assay
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Simple and sensitive progesterone detection in human serum using a CdSe/ZnS quantum dot-based direct binding assay Simple and sensitive progesterone detection in human serum using a CdSe/ZnS quantum dot-based direct binding assay](/preview/png/7558246.png)
چکیده انگلیسی
In this study, we developed a CdSe/ZnS quantum dot (QD)-based immunoassay for use in determining the presence of progesterone (P4) in human serum. Hydrophilic QDs were conjugated to anti-progesterone antibody (P4Ab) via ethyl-3-(dimethylaminopropyl)carbodiimide (EDC) and N-hydroxysuccinimide (NHS) as coupling reagents. After purification, the P4Ab-QD conjugates were immobilized onto the wells of a 96-well microtiter plate, and a direct-binding immunoassay based on the binding of P4 to immobilized P4Ab-QD conjugates had a detection limit of 0.21Â ng/ml and a sensitivity of 1.37Â ng/ml, with a linear range of 0.385 to 4.55Â ng/ml. The proposed immunoassay was successfully used to determine the P4 concentration in real human serum, and the results showed a good correlation with the accredited radioimmunoassay (RIA).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 483, 15 August 2015, Pages 54-61
Journal: Analytical Biochemistry - Volume 483, 15 August 2015, Pages 54-61
نویسندگان
Sung-Duk Oh, Hong Dinh Duong, Jong Il Rhee,