کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7558549 1491375 2015 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A set of external reference controls/probes that enable quality assurance between different microarray platforms
ترجمه فارسی عنوان
مجموعه ای از کنترل های ارجاع خارجی / پروب که تضمین کیفیت بین سیستم های مختلف میکروارگانی را فراهم می کند
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
RNA external standards, although important to ensure equivalence across many microarray platforms, have yet to be fully implemented in the research community. In this article, a set of unique RNA external standards (or RNA standards) and probe pairs that were added to total RNA in the samples before amplification and labeling are described. Concentration-response curves of RNA external standards were used across multiple commercial DNA microarray platforms and/or quantitative real-time polymerase chain reaction (RT-PCR) and next-generation sequencing to identify problematic assays and potential sources of variation in the analytical process. A variety of standards can be added in a range of concentrations spanning high and low abundances, thereby enabling the evaluation of assay performance across the expected range of concentrations found in a clinical sample. Using this approach, we show that we are able to confirm the dynamic range and the limit of detection for each DNA microarray platform, RT-PCR protocol, and next-generation sequencer. In addition, the combination of a series of standards and their probes was investigated on each platform, demonstrating that multiplatform calibration and validation is possible.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 472, 1 March 2015, Pages 75-83
نویسندگان
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